Studies » Effect of EnergoIod Preparation on Induction of Humoral and Cell-Mediated Immune Response of Cultivated Human Lymphocytes
Effect of EnergoIod Preparation on Induction of Humoral and Cell-Mediated Immune Response of Cultivated Human Lymphocytes
1. Effect of the preparation on induction of humoral immune response of human tonsillar lymphocytes to Y. Enterocolitica and Staphylococus Aureus in vitro.
We conducted studies with human tonsils (obtained after surgical treatment of tonsillitis). Results of the studies shown that immunostimulating effect of the preparation on induction of humoral immune response is shown with dilution rate of 1:10 - 1:100. Higher dilution rate (1:200 and 1:33) did not produce immunomodulating effect and did not differ from control values. The preparation diluted with dilution rate of 1:10 and 1:20 shows evident immunomodulating effect.
Based upon the results of the studies we can conclude that this preparation diluted with dilution rate of 1:10-1:100 has evident immunopotentiating effect expressed in enhancement of induction of humoral immune response of cultivated human lymphocytes to antigens Yersinia Enterferocolitica and Staphilococus Aureus.
2. Effect of the studied preparation on induction of cell-mediated immune response of tonsillar lymphocytes to live bacteria Y. Enterocolitica and Staphylococus Aureus in vitro.
Antibacterial activity of tonsillar lymphocytes against Y. Enterocolitica (strain 03 and 09) and S. Aureus (strain 5828) was estimated with application of a special method. Biochemical activity of standard bacterial strains was accepted as 100%.
This preparation diluted with dilution rate of 1:10, 1:20 and 1:50 stimulates antibacterial activity of cultivated lymphocytes against Y. Enterocolitica (strain 03 and 09). The highest efficiency was observed with dilution rate of 1:20 and 1:50.
Similar results were obtained in the study of antibacterial activity of cultivated lymphocytes against S. Aureus 5828. The preparation diluted with dilution rate of 1:10 and 1:50 stimulates antibacterial activity of cultivated lymphocytes. The highest efficiency was observed with dilution rate of 1:20 and 1:50. Antibacterial activity of cultivated lymphocytes was higher in the presence of the preparation than in the presence of polyclonal stimulator of immunocompetent cells.
Based upon the results of the studies we can conclude that this preparation diluted with dilution rate of 1:10-1:50 stimulates antibacterial activity of lymphocytes against pathogenic (S. Aureus) and opportunistic pathogenic (Y. Enterocolitica) strains of microorganisms expressed in suppression of biochemical activity of bacteria in the presence of cultivated lymphocytes treated with the preparation.
3. Effect of the studied preparation on survivability of human lymphocytes and complement-dependent haemolysis of sheep erythrocytes.
In the course of observations it was established that in the dynamics of the preparation’s action there is a short lag period lasting for 40 minutes. In this time period aggregation of cells and formation of suspension was observed, as well as separation of phases, cell residue - culture medium.
Results of our studies of cytotoxic effect of the preparation on lymphocytes shown that incubation of cells together with the preparation diluted with dilution rate of 1:10 -1:300 during 30 minutes did not result in death of cells in the culture in comparison with uncultivated lymphocytes. On this basis we suppose that in this period the preparation interacts with membranous cell structures without showing cytotoxic effect. In order to study this process in dynamics we conducted studies of sensitivity of sheep erythrocytes treated with the preparation to complement dependent (membrane-associated) haemolysis. We used only high concentrations of the preparation (dilution rate 1:10) and 10 min, 30 min and 90 min - interval of he preparation’s action.
The studied preparation does not have cytotoxic properties, but it has evident membrane-tropic effect expressed in the dynamics of the preparation’s interaction with plasma membranes of cells.