Studies » Toxicity of EnergoIod preparation upon application to skin and mucosa
Toxicity of EnergoIod preparation upon application to skin and mucosa
Local action of the preparation was studied in rats and rabbits. Irritative action was estimated based upon appearance of hyperemia, edema or thickening of skin fold in the place of application.
In study of local action of EnergoIod on mucosal membranes 1-2 drops of the preparation diluted with physiological salt solution in different concentrations were applied once to conjunctival sac of rabbits and rats. During the first minute nasolacrimal duct was pressed down. Then researchers registered appearance and intensity of hyperemia and edema, injection of vessels in sclera and cornea, condition of eyelids. Effect of the preparation on mucosal membranes was assessed by the character of conjunctivitis and keratitis.
Obtained data are shown in table 1.
According to table 1 the preparation does not induce irritation of skin, slight irritative effect was observed in rats and rabbits.
Таблица 1Toxic and allergenic effect of the studied drug.Dilution rate | Application to skin |
baseline | 1:1 | 1:10 | 1:20 | 1:30 | 1:50 | 1:100 |
Rat | + | + | - | - | - | - | - |
Rabbit | + | + | - | - | - | - | - |
Iodine concentration 0.008 gml.
Note:
+ hyperemia;
++ thickening of skin fold;
+++ edema. |
Dilution rate | Application to skin |
baseline | 1:1 | 1:10 | 1:20 | 1:30 | 1:50 | 1:100 |
Rat | ++++ | +++ | + | - | - | - | - |
Rabbit | ++++ | +++ | + | - | - | - | - |
Note:
+ superficial conjunctivitis;
++ profound conjunctivitis;
+++ keratitis;
++++ mucosal burn
|
In long-term studies (3 months)
EnergoIod was applied to skin for 4 hours daily. No irritative effects were observed, quite to the contrary skin became elastic. Skin- resorptive effect was not observed as well.
The studied preparation EnergoIod diluted with physiological salt solution with dilution rate from 1:20 to 1:100 did not induce irritation of mucosal membranes.