Studies » Pharmacotoxicology of EnetgoIod Preparation
Pharmacotoxicology of EnetgoIod Preparation
The preparation’s toxicity upon single-dose and multiple-dose administration was studied in various laboratory animals with the aim of determining its safety. In the course of those studies main acute toxicity indicators were determined (LD 100, LD 50 and MTD) with intravenious, oral and intraperitoneal administration of the preparation to mice and rats.
It was established that LD 50 for mice and rats with intravenous administration of the preparation is 3.8 and 5.3 ml/kg correspondingly, with intraperitoneal administration - 5.62 and 4.65 ml/kg, with oral administration - 31.5 ml/kg and more. Cumulative index after 2 weeks of intraperitoneal administration of the preparation to rats was equal to 4.2.
Analysis of the results of acute toxicity study didn’t revealed any tangible differences between sensitivity of mice and rats to the preparation. Parameters of toxicity for male and female animals were equal. The main symptoms of toxicity noticed after use of sublethal doses of the preparation were the following: short-term agitation gradually turning into depression and deferred reactions. As a whole test results are indicative of relatively low toxicity of the preparation and its reduced accumulative ability upon repeated use.
Toxicological studies with repeated administration of the preparation were conducted with three species of laboratory animals (rats, rabbits and dogs). The preparation was administered in therapeutic doses and in doses 5 and 10 times higher than daily average dose recommended for people. It was determined that the specified doses of the preparation do not affect significantly general state, behavior and body temperature of animals.
No any statistically significant changes were noticed in their haemopoiesis, biochemical characteristics of urine, as well as activity of enzymes in blood serum, content of crude protein, bilirubin, cholesterol, urea, creatinine and glucose, characterizing functional state of liver, kidneys and major types of intermediary metabolism. No any disorders in activity of cardiovascular and central nervous systems were noticed. Ophthalmological studies didn’t reveal any deviations as well.
Pathomorphological studies conducted in the end of the experiment didn’t reveal any changes in the relative mass of viscera or significant disturbances in separate organs and systems.
Intraperitoneal administration of high doses of the preparation causes transient decrease in body weight of rats and changes in certain hematological indicators.
Thus results of toxicological studies with various laboratory animals are indicative of safety of repeated use of EnergoIod.